D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P251M

Expression Data

Yield (mg/mL): 0.4995

Kinetic Assay Data

**slope of P251M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.34E-02	1.29E-02	1.43E-02
B	25.00	8.85E-03	1.10E-02	1.07E-02
C	8.33	9.00E-03	8.55E-03	8.40E-03
D	2.78	7.05E-03	7.65E-03	6.00E-03
E	0.93	4.65E-03	3.30E-03	3.75E-03
F	0.31	1.35E-03	1.50E-03	1.65E-03
G	0.10	1.05E-03	1.05E-03	9.00E-04
H	0.03	7.50E-04	6.00E-04	7.50E-04

Yield (mg/mL): 0.4995 Dilution Factor: 100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by Georgina001 and last updated on 2024-11-22 13:17:55.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.09E-01	3.37E-01	3.18E-01
B	25.00	2.70E-01	2.71E-01	2.72E-01
C	8.33	2.25E-01	2.34E-01	2.33E-01
D	2.78	1.91E-01	1.93E-01	1.92E-01
E	0.93	7.59E-02	1.13E-01	8.67E-02
F	0.31	3.72E-02	3.69E-02	2.99E-02
G	0.10	1.47E-02	1.35E-02	1.19E-02
H	0.03	6.00E-04		6.00E-04

Yield (mg/mL): 5.032 Dilution Factor: 100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:39:33.

Thermostability Assay Data

**slope of P251M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3		0	0
C	45.7	0	0	0
D	42.4	7.50E-04	7.50E-04
E	37.7		9.00E-04	9.00E-04
F	33.6	2.10E-03	2.10E-03	2.25E-03
G	31.3	2.70E-03	2.85E-03
H	30.0	2.55E-03	2.70E-03	2.85E-03

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by Georgina001 and last updated on 2024-11-22 13:24:47.

Reference Data for WT

**slope of P251M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04	6.00E-04
B	48.3	6.00E-04	6.00E-04	6.00E-04
C	45.7	9.00E-04	9.00E-04	7.50E-04
D	42.4	2.10E-02	2.10E-02	2.07E-02
E	37.7	1.60E-01	1.80E-01	1.81E-01
F	33.6	1.88E-01	1.88E-01	1.72E-01
G	31.3	1.76E-01	1.64E-01	1.51E-01
H	30.0	1.51E-01	1.47E-01	1.46E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:03:38.

Design Data

Rosetta Score for P251M: 165.372

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.