Raw Data for BglB Variant P251S

Expression Data

SDS-PAGE
 3.412

Kinetic Assay Data

slope of P251S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2367 0.2294 0.2588
B 25.00 0.1653 0.1785 0.1856
C 8.33 0.1584 0.1713 0.1713
D 2.78 0.09825 0.1253 0.1263
E 0.93 0.04305 0.04275 0.0447
F 0.31 0.0177 0.01935 0.01695
G 0.10 0.00675 0.0075 0.0072
H 0.03 0 0
 3.412      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by gbarr112 and last updated on 2024-11-22 13:14:18.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.36E-01 3.75E-01
B 25.00 2.97E-01 2.96E-01 3.02E-01
C 8.33 2.74E-01 2.76E-01 2.77E-01
D 2.78 1.63E-01 1.86E-01 1.64E-01
E 0.93 6.99E-02 7.02E-02 8.04E-02
F 0.31 3.14E-02 3.60E-02 2.96E-02
G 0.10 1.01E-02 1.11E-02 1.35E-02
H 0.03 0 0
 5.215      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by katherineg1230 and last updated on 2024-11-22 13:36:46.


Thermostability Assay Data

slope of P251S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00045 0.00075
B 48.3 0.0006 0.00075
C 45.7 0.0006 0.0006002
D 42.4 0.00105 0.00105 0.0012
E 37.7 0.01845 0.0171 0.02085
F 33.6 0.0816 0.08235 0.0843
G 31.3 0.0924 0.09165 0.08655
H 30.0 0.0879 0.0876 0.1039

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by gbarr112 and last updated on 2024-11-22 13:34:01.


Reference Data for WT

slope of P251S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.50E-04
D 42.4 1.65E-02 1.56E-02 1.61E-02
E 37.7 1.91E-01 1.81E-01 1.90E-01
F 33.6 2.26E-01 2.25E-01 2.12E-01
G 31.3 1.83E-01 1.88E-01 1.75E-01
H 30.0 1.94E-01 1.84E-01 1.78E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by katherineg1230 and last updated on 2024-11-22 13:37:23.


Design Data

-6.084

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.