D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P251S

Expression Data

Yield (mg/mL): 2.955

Kinetic Assay Data

**slope of P251S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.61E-01	1.57E-01	1.65E-01
B	25.00	1.38E-01	1.50E-01	1.46E-01
C	8.33	1.03E-01	1.05E-01	1.19E-01
D	2.78	9.33E-02	1.02E-01	9.63E-02
E	0.93	4.19E-02	4.44E-02	4.62E-02
F	0.31	1.64E-02	1.73E-02	1.62E-02
G	0.10	5.70E-03	8.55E-03	7.20E-03
H	0.03	4.50E-04	6.00E-04

Yield (mg/mL): 2.955 Dilution Factor: 100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Nfernandez and last updated on 2024-11-22 13:09:58.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.09E-01	3.37E-01	3.18E-01
B	25.00	2.70E-01	2.71E-01	2.72E-01
C	8.33	2.25E-01	2.34E-01	2.33E-01
D	2.78	1.91E-01	1.93E-01	1.92E-01
E	0.93	7.59E-02	1.13E-01	8.67E-02
F	0.31	3.72E-02	3.69E-02	2.99E-02
G	0.10	1.47E-02	1.35E-02	1.19E-02
H	0.03	6.00E-04		6.00E-04

Yield (mg/mL): 5.032 Dilution Factor: 100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:39:33.

Thermostability Assay Data

**slope of P251S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04	6.00E-04	6.00E-04
B	48.3	0	0	0
C	45.7	7.50E-04	6.00E-04	6.00E-04
D	42.4	7.50E-04	7.50E-04
E	37.7	1.41E-02	1.35E-02	1.32E-02
F	33.6	6.11E-02	7.35E-02	7.07E-02
G	31.3	7.53E-02	7.04E-02	7.25E-02
H	30.0	6.36E-02	5.91E-02	7.55E-02

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Nfernandez and last updated on 2024-11-22 13:11:38.

Reference Data for WT

**slope of P251S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04	6.00E-04
B	48.3	6.00E-04	6.00E-04	6.00E-04
C	45.7	9.00E-04	9.00E-04	7.50E-04
D	42.4	2.10E-02	2.10E-02	2.07E-02
E	37.7	1.60E-01	1.80E-01	1.81E-01
F	33.6	1.88E-01	1.88E-01	1.72E-01
G	31.3	1.76E-01	1.64E-01	1.51E-01
H	30.0	1.51E-01	1.47E-01	1.46E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:03:38.

Design Data

Rosetta Score for P251S: -6.084

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.