Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 4.121

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.07E-01	3.02E-01	2.72E-01
	B	25.00	2.52E-01	2.48E-01	2.46E-01
	C	8.33	2.34E-01	2.43E-01	2.25E-01
	D	2.78	1.20E-01	1.32E-01	1.35E-01
	E	0.93	5.48E-02	5.48E-02	5.48E-02
	F	0.31	2.07E-02	2.12E-02	2.22E-02
	G	0.10	7.50E-03	7.05E-03	7.35E-03
	H	0.03	0	7.50E-04	4.50E-04

Yield (mg/mL): 4.121     Dilution Factor:  100×

Download these data

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:08:38.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04		4.50E-04
	B	48.3		6.00E-04	6.00E-04
	C	45.7	9.00E-04	9.00E-04	1.05E-03
	D	42.4		1.61E-02	1.59E-02
	E	37.7	1.27E-01	1.39E-01	1.29E-01
	F	33.6	1.67E-01	1.46E-01	1.58E-01
	G	31.3	1.74E-01	1.56E-01	1.51E-01
	H	30.0	1.51E-01	1.41E-01

Download these data

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:12:21.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.