Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.032

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.09E-01	3.37E-01	3.18E-01
	B	25.00	2.70E-01	2.71E-01	2.72E-01
	C	8.33	2.25E-01	2.34E-01	2.33E-01
	D	2.78	1.91E-01	1.93E-01	1.92E-01
	E	0.93	7.59E-02	1.13E-01	8.67E-02
	F	0.31	3.72E-02	3.69E-02	2.99E-02
	G	0.10	1.47E-02	1.35E-02	1.19E-02
	H	0.03	6.00E-04		6.00E-04

Yield (mg/mL): 5.032     Dilution Factor:  100×

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Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:39:33.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	7.50E-04	6.00E-04
	B	48.3	6.00E-04	6.00E-04	6.00E-04
	C	45.7	9.00E-04	9.00E-04	7.50E-04
	D	42.4	2.10E-02	2.10E-02	2.07E-02
	E	37.7	1.60E-01	1.80E-01	1.81E-01
	F	33.6	1.88E-01	1.88E-01	1.72E-01
	G	31.3	1.76E-01	1.64E-01	1.51E-01
	H	30.0	1.51E-01	1.47E-01	1.46E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:03:38.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.