D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V314W

Expression Data

Yield (mg/mL): 6.071

Kinetic Assay Data

**slope of V314W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.77E-01	2.72E-01	2.96E-01
B	25.00	2.67E-01	2.63E-01	2.51E-01
C	8.33	1.84E-01	1.92E-01	1.90E-01
D	2.78	1.10E-01	1.16E-01	1.12E-01
E	0.93	4.74E-02	5.34E-02	5.01E-02
F	0.31	1.82E-02	1.79E-02	1.70E-02
G	0.10	6.15E-03	5.85E-03	7.20E-03
H	0.03		7.50E-04	6.00E-04

Yield (mg/mL): 6.071 Dilution Factor: 100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 09:24:29.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.69E-01	3.76E-01	3.61E-01
B	25.00	2.82E-01	3.06E-01	2.98E-01
C	8.33	2.14E-01	2.26E-01	2.24E-01
D	2.78	1.21E-01	1.31E-01	1.34E-01
E	0.93	5.94E-02	4.91E-02	5.97E-02
F	0.31	1.70E-02	1.71E-02	1.80E-02
G	0.10	6.00E-03	6.45E-03	7.05E-03
H	0.03

Yield (mg/mL): 6.75 Dilution Factor: 100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:39:26.

Thermostability Assay Data

**slope of V314W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0
B	48.3	9.00E-04	6.00E-04	4.50E-04
C	45.7	1.20E-03	6.00E-04	7.50E-04
D	42.4	1.95E-03	1.05E-03	1.50E-03
E	37.7	6.05E-02	5.63E-02	5.75E-02
F	33.6	1.27E-01	1.30E-01	1.53E-01
G	31.3	1.27E-01	1.32E-01	1.35E-01
H	30.0	1.41E-01	1.35E-01	1.42E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:54:58.

Reference Data for WT

**slope of V314W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0
B	48.3
C	45.7		7.50E-04	9.00E-04
D	42.4	1.71E-02	1.47E-02	1.38E-02
E	37.7	1.35E-01	1.37E-01	1.33E-01
F	33.6	1.42E-01	1.52E-01	1.56E-01
G	31.3	1.59E-01	1.53E-01	1.55E-01
H	30.0	1.69E-01	1.60E-01	1.66E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:39:48.

Design Data

Rosetta Score for V314W: 7.696

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.