Raw Data for BglB Variant V314E

Expression Data

SDS-PAGE
 6.378

Kinetic Assay Data

slope of V314E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.53E-01 2.58E-01
B 25.00 2.08E-01 1.96E-01 2.15E-01
C 8.33 1.67E-01 1.63E-01 1.62E-01
D 2.78 1.46E-01 1.57E-01 1.47E-01
E 0.93 5.25E-02 6.45E-02 6.32E-02
F 0.31 2.00E-02 2.45E-02 2.18E-02
G 0.10 8.40E-03 1.04E-02 8.85E-03
H 0.03 4.50E-04 6.00E-04
 6.378      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by joyceb0418 and last updated on 2024-11-22 08:48:01.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.63E-01 3.70E-01 3.60E-01
B 25.00 3.03E-01 2.99E-01 3.03E-01
C 8.33 2.61E-01 2.44E-01 2.33E-01
D 2.78 1.81E-01 1.97E-01 2.19E-01
E 0.93 9.44E-02 8.88E-02 8.96E-02
F 0.31 3.45E-02 3.18E-02 3.00E-02
G 0.10 1.17E-02 1.14E-02 1.23E-02
H 0.03
 2.62      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Zariahnasir and last updated on 2024-11-22 08:47:09.


Thermostability Assay Data

slope of V314E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.50E-04 6.00E-04
B 48.3 6.00E-04 6.00E-04
C 45.7 0 0 0
D 42.4 2.40E-03 2.55E-03 2.25E-03
E 37.7 9.20E-02 8.79E-02 8.10E-02
F 33.6 1.53E-01 1.53E-01 1.54E-01
G 31.3 1.45E-01 1.58E-01 1.42E-01
H 30.0 1.60E-01 1.45E-01 1.41E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by joyceb0418 and last updated on 2024-11-22 09:03:44.


Reference Data for WT

slope of V314E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 6.00E-04
B 48.3 4.50E-04 4.50E-04
C 45.7 6.00E-04 6.00E-04
D 42.4 1.92E-02 1.80E-02 1.88E-02
E 37.7 2.00E-01 1.92E-01 1.92E-01
F 33.6 2.14E-01 2.16E-01 2.11E-01
G 31.3 2.30E-01 2.23E-01 2.09E-01
H 30.0 2.14E-01 2.12E-01 2.11E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Zariahnasir and last updated on 2024-11-22 08:48:45.


Design Data

2.841

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.