Raw Data for BglB Variant V314M

Expression Data

SDS-PAGE
 7.63

Kinetic Assay Data

slope of V314M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.77E-01 3.98E-01 3.93E-01
B 25.00 2.85E-01 3.13E-01 3.15E-01
C 8.33 2.27E-01 2.27E-01 2.29E-01
D 2.78 1.26E-01 1.18E-01 1.35E-01
E 0.93 5.28E-02 5.25E-02 5.46E-02
F 0.31 1.94E-02 2.12E-02 2.18E-02
G 0.10 7.80E-03 8.10E-03 8.10E-03
H 0.03 0 0 0
 7.63      100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by Dvegafold and last updated on 2024-11-22 08:44:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.69E-01 3.76E-01 3.61E-01
B 25.00 2.82E-01 3.06E-01 2.98E-01
C 8.33 2.14E-01 2.26E-01 2.24E-01
D 2.78 1.21E-01 1.31E-01 1.34E-01
E 0.93 5.94E-02 4.91E-02 5.97E-02
F 0.31 1.70E-02 1.71E-02 1.80E-02
G 0.10 6.00E-03 6.45E-03 7.05E-03
H 0.03
 6.75      100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:39:26.


Thermostability Assay Data

slope of V314M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 4.50E-04 4.50E-04
C 45.7 4.50E-04 6.00E-04
D 42.4 4.50E-03 3.45E-03 4.05E-03
E 37.7 1.40E-01 1.26E-01 1.16E-01
F 33.6 1.72E-01 1.65E-01 1.84E-01
G 31.3 1.73E-01 1.89E-01 1.78E-01
H 30.0 2.10E-01 1.85E-01

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by Dvegafold and last updated on 2024-11-22 09:00:55.


Reference Data for WT

slope of V314M A420 nm (min−1)
T (°C) 1 2 3
A 50.0
B 48.3
C 45.7 7.50E-04 9.00E-04
D 42.4 1.71E-02 1.47E-02 1.38E-02
E 37.7 1.35E-01 1.37E-01 1.33E-01
F 33.6 1.42E-01 1.52E-01 1.56E-01
G 31.3 1.59E-01 1.53E-01 1.55E-01
H 30.0 1.69E-01 1.60E-01 1.66E-01

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by RachelleRevilla and last updated on 2024-11-22 08:39:48.


Design Data

4.267

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.