Raw Data for BglB Variant V314Q

Expression Data

Yield (mg/mL): 6.592

Kinetic Assay Data

slope of V314Q A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.23E-01	3.51E-01	3.42E-01
	B	25.00	2.65E-01	2.66E-01	2.64E-01
	C	8.33	1.97E-01	2.05E-01	2.16E-01
	D	2.78	1.58E-01	2.04E-01	1.95E-01
	E	0.93	6.48E-02	5.82E-02	7.07E-02
	F	0.31	2.52E-02	2.64E-02	3.00E-02
	G	0.10	1.20E-02	1.05E-02	1.01E-02
	H	0.03	6.00E-04	6.00E-04

Yield (mg/mL): 6.592     Dilution Factor:  100×

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by kayleerubio and last updated on 2024-11-22 08:43:04.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of V314Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	6.00E-04	6.00E-04
	B	48.3	0		0
	C	45.7	7.50E-04	6.00E-04	6.00E-04
	D	42.4	3.30E-03	3.30E-03	3.15E-03
	E	37.7	1.28E-01	1.25E-01	1.25E-01
	F	33.6	1.69E-01	2.03E-01	1.68E-01
	G	31.3	1.86E-01	1.70E-01	1.58E-01
	H	30.0	1.80E-01	1.70E-01	1.77E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by kayleerubio and last updated on 2024-11-22 08:43:44.

Reference Data for WT

slope of V314Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04		6.00E-04
	B	48.3	4.50E-04		4.50E-04
	C	45.7	6.00E-04		6.00E-04
	D	42.4	1.92E-02	1.80E-02	1.88E-02
	E	37.7	2.00E-01	1.92E-01	1.92E-01
	F	33.6	2.14E-01	2.16E-01	2.11E-01
	G	31.3	2.30E-01	2.23E-01	2.09E-01
	H	30.0	2.14E-01	2.12E-01	2.11E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Zariahnasir and last updated on 2024-11-22 08:48:45.

Design Data

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.