Raw Data for BglB Variant S203A

Expression Data

SDS-PAGE
No expression

Kinetic Assay Data

slope of S203A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.27E+02 3.32E+02 3.43E+02
B 25.00 2.56E+02 2.52E+02 2.66E+02
C 8.33 1.88E+02 1.82E+02 1.84E+02
D 2.78 1.33E+02 1.16E+02 1.23E+02
E 0.93 5.72E+01 5.20E+01 5.37E+01
F 0.31 2.14E+01 1.99E+01 1.95E+01
G 0.10 5.00E-02 4.00E-02
H 0.03 3.00E-02 4.00E-02 3.00E-02
 0.698      10×

Protein purified on and assayed on .
Data uploaded by rbreitlow and last updated on 2024-12-11 16:46:57.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.90E-01 4.56E-01 3.00E-02
B 25.00 9.00E-02 3.10E-01 9.70E-01
C 8.33 2.10E-01 2.50E-01
D 2.78 1.90E-01 2.50E-01 3.00E-02
E 0.93 9.00E-02 1.40E-01 3.00E-02
F 0.31 1.49E-01 5.00E-02
G 0.10 3.00E-02 5.00E-02
H 0.03 2.92E+00 1.09E+00 1.70E-01
 0.364      10×

Protein purified on 10/11/24 and assayed on 11/5/24.
Data uploaded by rbreitlow and last updated on 2024-12-11 13:41:27.


Thermostability Assay Data

slope of S203A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-02 7.00E-02
B 48.3 1.20E-01 1.20E-01
C 45.7 8.00E-02 5.00E-02
D 42.4 6.90E-02 6.00E-02
E 37.7 1.20E-01 1.40E-01
F 33.6 5.00E-02 4.00E-01
G 31.3 8.90E-02 6.00E-02 1.10E-01
H 30.0 9.00E-02 7.00E-02

Protein purified on 11/4/24 and assayed on 11/19/24.
Data uploaded by rbreitlow and last updated on 2024-12-11 13:37:56.


Reference Data for WT

slope of S203A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.70E-01 1.70E-01
B 48.3 2.40E-01 2.30E-01 2.50E-01
C 45.7 1.80E-01 2.78E-01
D 42.4
E 37.7 1.80E-01 4.00E-02
F 33.6 9.00E-02 6.00E-02
G 31.3 5.00E-02 7.00E-02
H 30.0 6.00E-02 2.00E-02

Protein purified on 11/4/24 and assayed on 11/19/24.
Data uploaded by rbreitlow and last updated on 2024-12-11 13:42:40.


Design Data

-1.882

Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.