D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E154I

Yield (mg/mL): 0.877

**slope of E154I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.71E+01	4.10E+01	3.76E+01
B	25.00	3.13E+01	3.06E+01	3.27E+01
C	8.33	2.59E+01	2.48E+01
D	2.78	1.89E+01	1.67E+01	1.76E+01
E	0.93	8.53E+00	7.48E+00	1.04E+01
F	0.31		1.72E+00	1.32E+00
G	0.10	2.00E-02	5.00E-02	3.00E-02
H	0.03	2.00E-02	3.00E-02	4.00E-02

Yield (mg/mL): 0.877 Dilution Factor: 10×

Protein purified on and assayed on .
Data uploaded by JorenOlson and last updated on 2024-12-05 12:03:24.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	196.518	200.219	201.575
B	25.00	153.305	157.123	152.092
C	8.33	124.35	132.67	130.08
D	2.78	83.78	83.813	87.229
E	0.93	41.339	43.794	48.194
F	0.31	15.089	16.177	19.668
G	0.10	0.06		0.06
H	0.03	0.03	0.05	0.06

Yield (mg/mL): 0.327 Dilution Factor: 10×

Protein purified on and assayed on .
Data uploaded by ChaseM and last updated on 2024-12-05 12:04:48.

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.