Raw Data for BglB Variant W81C

Expression Data

SDS-PAGE
 0.164

Kinetic Assay Data

slope of W81C A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.97E-01 1.88E-01 1.75E-01
B 25.00 8.90E-02 1.04E-01 9.90E-02
C 8.33 9.10E-02 7.70E-02 7.20E-02
D 2.78 7.20E-02 6.20E-02 5.80E-02
E 0.93 5.90E-02 5.30E-02 4.90E-02
F 0.31 5.00E-02 4.70E-02 4.50E-02
G 0.10 4.70E-02 4.50E-02 4.60E-02
H 0.03 4.60E-02 4.40E-02 4.10E-02
 0.164      10×

Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by Emma27 and last updated on 2024-12-12 07:34:19.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.85E+00 3.82E+00 3.64E+00
B 25.00 3.85E+00 3.69E+00 3.38E+00
C 8.33 3.51E+00 3.25E+00 2.77E+00
D 2.78 2.55E+00 2.30E+00 1.79E+00
E 0.93 1.37E+00 1.04E+00 6.92E-01
F 0.31 6.51E-01 5.09E-01 3.63E-01
G 0.10 2.55E-01 2.36E-01
H 0.03 4.20E-02 4.20E-02 4.60E-02
 1.286      10×

Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by Autumnbayerl and last updated on 2024-12-05 09:17:55.


Thermostability Assay Data

slope of W81C A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-02 0
B 48.3 7.00E-02 8.00E-02
C 45.7 6.00E-02 1.09E-01 0
D 42.4 2.90E-01 1.78E-01 2.50E-01
E 37.7 4.35E+00 4.00E+00
F 36.6 4.88E+00 5.72E+00
G 31.3 4.76E+00 3.12E+00 7.37E+00
H 30.0 2.59E+00 5.04E+00 7.77E+00

Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by Autumnbayerl and last updated on 2024-12-12 07:47:24.


Reference Data for WT

slope of W81C A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.14 1.30E-01
B 48.3 2.68E-01 3.10E-01
C 45.7 1.32E+00 1.11E+00
D 42.4 2.17E+01 2.35E+01 2.40E+01
E 37.7 1.22E+02 1.72E+02 1.47E+02
F 33.6 1.89E+02 1.76E+02
G 31.3 1.39E+02 1.67E+02 1.38E+02
H 30.0 2.01E+02 2.00E+02

Protein purified on 11/7/2024 and assayed on 11/14/2014.
Data uploaded by Autumnbayerl and last updated on 2024-12-12 07:54:37.


Design Data

8.947

Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.