Raw Data for BglB Variant G23A

Expression Data

Yield (mg/mL): 0.73

Kinetic Assay Data

slope of G23A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1324	0.1499	0.1406
	B	25.00	0.1194	0.1269	0.1204
	C	8.33	0.09515	0.1002	0.1024
	D	2.78	0.1157	0.1148	0.08730
	E	0.93	0.04725	0.04595	0.05060
	F	0.31	0.01720	0.02010	0.02480
	G	0.10	0.008050	0.008750	0.009800
	H	0.03		0.0001500	0.0002000

Yield (mg/mL): 0.73     Dilution Factor:  100×

Download these data

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by ecornejo and last updated on 2024-11-20 15:00:16.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1935	0.1974	0.2006
	B	25.00	0.1839	0.1755	0.1746
	C	8.33	0.1570	0.1403	0.1394
	D	2.78	0.1276	0.1393	0.1317
	E	0.93	0.06295	0.06160	0.07165
	F	0.31	0.02480	0.02910	0.03175
	G	0.10	0.009800	0.009900	0.012500
	H	0.03	0.000200		0.000200

Yield (mg/mL): 1.07     Dilution Factor:  100×

Download these data

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by MuhammadK and last updated on 2024-11-20 15:24:44.

Thermostability Assay Data

slope of G23A A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.00E-04	5.00E-04	5.00E-04
	B	48.3	6.00E-04	5.00E-04	5.00E-04
	C	45.7	8.00E-04	8.00E-04	8.00E-04
	D	42.4	5.09E-02	4.48E-02	5.57E-02
	E	37.7	3.52E-01	3.29E-01	3.27E-01
	F	33.6	3.39E-01	3.45E-01	3.46E-01
	G	31.3	3.83E-01	3.75E-01	3.83E-01
	H	30.0	3.78E-01	3.60E-01	3.80E-01

Download these data

Protein purified on 10/30/24 and assayed on 10/30/24.
Data uploaded by ecornejo and last updated on 2024-11-20 15:16:11.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.