D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant G271D

Expression Data

Yield (mg/mL): 0.61

Kinetic Assay Data

**slope of G271D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.53E-01	1.58E-01	1.50E-01
B	25.00	1.25E-01	1.26E-01	1.18E-01
C	8.33	1.02E-01	1.08E-01	1.08E-01
D	2.78	1.08E-01	1.01E-01	8.32E-02
E	0.93	5.74E-02	6.78E-02	6.58E-02
F	0.31	2.46E-02	2.61E-02	2.79E-02
G	0.10	1.14E-02	1.19E-02	1.24E-02
H	0.03		2.50E-04	2.00E-04

Yield (mg/mL): 0.61 Dilution Factor: 100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by Darrylwwong and last updated on 2024-12-05 18:13:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1935	0.1974	0.2006
B	25.00	0.1839	0.1755	0.1746
C	8.33	0.1570	0.1403	0.1394
D	2.78	0.1276	0.1393	0.1317
E	0.93	0.06295	0.06160	0.07165
F	0.31	0.02480	0.02910	0.03175
G	0.10	0.009800	0.009900	0.012500
H	0.03	0.000200		0.000200

Yield (mg/mL): 1.07 Dilution Factor: 100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by MuhammadK and last updated on 2024-11-20 15:24:44.

Thermostability Assay Data

**slope of G271D A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	0	9.00E-04
B	48.3	6.00E-04	0	4.00E-04
C	45.7	7.00E-04	8.00E-04	9.00E-04
D	42.4	4.10E-02	4.79E-02	5.49E-02
E	37.7	1.96E-01	2.28E-01	2.01E-01
F	33.6	2.10E-01	1.90E-01	1.89E-01
G	31.3	2.39E-01	2.05E-01	2.01E-01
H	30.0	2.07E-01	2.02E-01	1.99E-01

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by Darrylwwong and last updated on 2024-11-20 15:18:20.

Design Data

Rosetta Score for G271D: 4.701

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.