Raw Data for BglB Variant N270G

Expression Data

SDS-PAGE
 0.98

Kinetic Assay Data

slope of N270G A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2484 0.2698 0.2656
B 25.00 0.2229 0.2328 0.2364
C 8.33 0.1806 0.1789 0.1983
D 2.78 0.136 0.1377 0.1698
E 0.93 0.0552 0.06485 0.0682
F 0.31 0.03055 0.0319 0.02995
G 0.10 0.01035 0.0118 0.01055
H 0.03 0.00025 0.00025
 0.98      100×

Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by NikhilKundu and last updated on 2024-11-20 15:24:55.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2862 0.2587 0.2615
B 25.00 0.2273 0.2437 0.2329
C 8.33 0.2244 0.2128 0.1853
D 2.78 0.1298 0.1306 0.1326
E 0.93 0.04780 0.05910
F 0.31 0.01795 0.02480 0.02665
G 0.10 0.008100 0.008050 0.009050
H 0.03 0.000250 0.000400 0
 1.21      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 15:02:47.


Thermostability Assay Data

slope of N270G A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006 0.0004 0.0005
B 48.3 0.0004001 0.0007 0.0006
C 45.7 0.001 0.0011 0.0011
D 42.4 0.0575 0.068 0.0719
E 37.7 0.2614 0.2296 0.2353
F 33.6 0.2489 0.2615 0.2574
G 31.3 0.246 0.2455 0.2436
H 30.0 0.277 0.248 0.2681

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by NikhilKundu and last updated on 2024-11-20 15:15:53.


Reference Data for WT

slope of N270G A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.000600 0.0015000 0
B 48.3 0.0005000 0.000500 0.0005000
C 45.7 0.001000 0.001100 0.001300
D 42.4 0.06780 0.06370 0.07000
E 37.7 0.2456 0.2398 0.2423
F 33.6 0.2568 0.2633 0.2403
G 31.3 0.2531 0.2537 0.2135
H 30.0 0.2613 0.2603 0.2774

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 15:14:11.


Design Data

3.159

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.