Raw Data for BglB Variant L122Q

Expression Data

SDS-PAGE
 0.2

Kinetic Assay Data

slope of L122Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 0.0419 0.0441 0.0412
C 8.33 0.0399 0.0347 0.0406
D 2.78 0.0250 0.0225 0.0226
E 0.93 0.01230 0.01190 0.01115
F 0.31 0.00560 0.00560 0.00505
G 0.10 0.002050 0.001650 0.002150
H 0.03 0 0 0
 0.2      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by Dale and last updated on 2024-11-20 15:14:46.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2862 0.2587 0.2615
B 25.00 0.2273 0.2437 0.2329
C 8.33 0.2244 0.2128 0.1853
D 2.78 0.1298 0.1306 0.1326
E 0.93 0.04780 0.05910
F 0.31 0.01795 0.02480 0.02665
G 0.10 0.008100 0.008050 0.009050
H 0.03 0.000250 0.000400 0
 1.21      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 15:02:47.


Thermostability Assay Data

slope of L122Q A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00140 0.0000000 0.000600
B 48.3 0.000500 0.0005000
C 45.7 0.000400 0.000000 0.001200
D 42.4 0.00110 0.00110 0.00090
E 37.7 0.0151 0.0165 0.0153
F 33.6 0.0329 0.0430 0.0347
G 31.3 0.0509 0.0386 0.0425
H 30.0 0.0420 0.0446 0.0398

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by Dale and last updated on 2024-11-20 15:20:39.


Reference Data for WT

slope of L122Q A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.000600 0.0015000 0
B 48.3 0.0005000 0.000500 0.0005000
C 45.7 0.001000 0.001100 0.001300
D 42.4 0.06780 0.06370 0.07000
E 37.7 0.2456 0.2398 0.2423
F 33.6 0.2568 0.2633 0.2403
G 31.3 0.2531 0.2537 0.2135
H 30.0 0.2613 0.2603 0.2774

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jazlyndias and last updated on 2024-11-20 15:14:11.


Design Data

7.994

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.