Raw Data for BglB Variant C38S

Expression Data

SDS-PAGE
 0.75

Kinetic Assay Data

slope of C38S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2902 0.3074 0.2904
B 25.00 0.2362 0.2442 0.2632
C 8.33 0.1770 0.2246 0.2138
D 2.78 0.1386 0.1406
E 0.93 0.05980 0.06415 0.06440
F 0.31 0.02160 0.02105 0.02270
G 0.10 0.008700 0.010450 0.008400
H 0.03 0 0.001150 0.000850
 0.75      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by MitaD and last updated on 2024-11-20 14:56:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3800 0.3190 0.3732
B 25.00 0.3007 0.3739 0.2900
C 8.33 0.2780 0.2833 0.2466
D 2.78 0.1580 0.1569 0.1614
E 0.93 0.07455 0.07365 0.08170
F 0.31 0.02520 0.02790 0.02520
G 0.10 0.00965 0.008600 0.011250
H 0.03 0 0
 0.95      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:20:19.


Thermostability Assay Data

slope of C38S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0008001 0.0007 0.0007
B 48.3 0.0007 0.0005 0.001
C 45.7 0.002 0.002
D 42.4 0.092 0.1065 0.1125
E 37.7 0.2886 0.2844 0.2777
F 33.6 0.2938 0.2926 0.2851
G 31.3 0.3009 0.2981 0.2966
H 30.0 0.2955 0.3044 0.277

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by MitaD and last updated on 2024-11-20 15:01:31.


Reference Data for WT

slope of C38S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.0030000 0.001100
B 48.3 0.0010000 0.001000 0.0012000
C 45.7 0.001400 0.001500 0.001300
D 42.4 0.05120 0.05190 0.06370
E 37.7 0.3834 0.3837 0.3719
F 33.6 0.3993 0.3857 0.4034
G 31.3 0.3932 0.4149 0.4068
H 30.0 0.4266 0.4082 0.4074

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:12:50.


Design Data

1.081

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.