Raw Data for BglB Variant Y268K

Expression Data

SDS-PAGE
 1.19

Kinetic Assay Data

slope of Y268K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4444 0.4461 0.4211
B 25.00 0.3441 0.3659 0.3570
C 8.33 0.4002 0.3035 0.3181
D 2.78 0.1833 0.2135 0.2333
E 0.93 0.09685 0.08720 0.10790
F 0.31 0.02990 0.03075 0.03390
G 0.10 0.013400 0.011450 0.012500
H 0.03 0 0 0
 1.19      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:09:16.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3800 0.3190 0.3732
B 25.00 0.3007 0.3739 0.2900
C 8.33 0.2780 0.2833 0.2466
D 2.78 0.1580 0.1569 0.1614
E 0.93 0.07455 0.07365 0.08170
F 0.31 0.02520 0.02790 0.02520
G 0.10 0.00965 0.008600 0.011250
H 0.03 0 0
 0.95      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:20:19.


Thermostability Assay Data

slope of Y268K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00100 0.0010000 0.001200
B 48.3 0.0012000 0.000900 0.0006000
C 45.7 0.002400 0.002400
D 42.4 0.03650 0.04050 0.03490
E 37.7 0.3766 0.3874 0.3738
F 33.6 0.4185 0.4181 0.4253
G 31.3 0.4385 0.4501 0.4425
H 30.0 0.4239 0.4335 0.4373

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:15:37.


Reference Data for WT

slope of Y268K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.0030000 0.001100
B 48.3 0.0010000 0.001000 0.0012000
C 45.7 0.001400 0.001500 0.001300
D 42.4 0.05120 0.05190 0.06370
E 37.7 0.3834 0.3837 0.3719
F 33.6 0.3993 0.3857 0.4034
G 31.3 0.3932 0.4149 0.4068
H 30.0 0.4266 0.4082 0.4074

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by maxineosoteo and last updated on 2024-11-20 15:12:50.


Design Data

9.243

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.