Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.65

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.30E-01	3.42E-01	3.12E-01
	B	25.00	2.46E-01	2.88E-01
	C	8.33	1.89E-01	1.75E-01
	D	2.78	1.75E-01	1.81E-01
	E	0.93
	F	0.31	7.41E-02	7.83E-02
	G	0.10	3.19E-02	3.39E-02
	H	0.03	1.41E-02	1.03E-02	1.74E-02

Yield (mg/mL): 0.65     Dilution Factor:  100×

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Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:13:44.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.70E-03	2.20E-03	2.80E-03
	B	48.3	9.00E-04		1.10E-03
	C	45.7	0	0
	D	42.4	4.38E-02	3.54E-02	3.15E-02
	E	37.7	3.62E-01	3.41E-01	3.30E-01
	F	33.6	3.34E-01	3.39E-01	3.85E-01
	G	31.3	3.90E-01	3.82E-01	4.13E-01
	H	30.0	3.52E-01	4.08E-01	3.43E-01

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Protein purified on 10/23/24 and assayed on 11/6/24.
Data uploaded by Mehek and last updated on 2024-11-20 15:31:52.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.