D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L272F

Expression Data

Yield (mg/mL): 1.23

Kinetic Assay Data

**slope of L272F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		0.0011	0.0010
C	8.33	7.50E-04	0.0006	0.0007
D	2.78	8.00E-04	0.0006	0.0006
E	0.93	4.00E-04	0.00045	0.00035
F	0.31	3.00E-04	0.00025
G	0.10	6.50E-04	0.000200
H	0.03	6.00E-04	0.000250	0.001150

Yield (mg/mL): 1.23 Dilution Factor: 100×

Protein purified on 10/29/24 and assayed on 11/16/24.
Data uploaded by mbahekar and last updated on 2024-11-20 12:17:30.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.28E-01	5.14E-01	5.20E-01
B	25.00	4.31E-01	4.06E-01	4.27E-01
C	8.33	3.86E-01	3.60E-01	3.65E-01
D	2.78	2.23E-01	2.25E-01	2.29E-01
E	0.93	1.04E-01	1.02E-01	1.05E-01
F	0.31	3.98E-02	3.95E-02	4.15E-02
G	0.10	1.39E-02	1.41E-02	1.30E-02
H	0.03		7.00E-04	8.50E-04

Yield (mg/mL): 1.59 Dilution Factor: 100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:36:41.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of L272F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.0012000	0.000600
B	48.3	0.0011		0.0009000
C	45.7	0.0011	0.001100	0.001200
D	42.4	0.0791	0.09140	0.11770
E	37.7	0.6272	0.5683	0.5244
F	33.6	0.6226	0.6248	0.6226
G	31.3	0.6319	0.6288	0.6255
H	30.0	0.628	0.6750	0.6181

Protein purified on 11/6/2024 and assayed on 11/6/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:53:49.

Design Data

Rosetta Score for L272F: 80.947

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.