D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N421D

Expression Data

Yield (mg/mL): 0.69

Kinetic Assay Data

**slope of N421D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.93E-01	1.87E-01	1.60E-01
B	25.00	1.51E-01	1.56E-01	1.30E-01
C	8.33	1.16E-01	1.35E-01	1.18E-01
D	2.78	9.83E-02	7.69E-02	7.73E-02
E	0.93	3.76E-02	3.56E-02	3.79E-02
F	0.31	1.62E-02	1.38E-02	1.01E-02
G	0.10	5.35E-03	5.55E-03	4.90E-03
H	0.03		0	0

Yield (mg/mL): 0.69 Dilution Factor: 100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:09:48.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.28E-01	5.14E-01	5.20E-01
B	25.00	4.31E-01	4.06E-01	4.27E-01
C	8.33	3.86E-01	3.60E-01	3.65E-01
D	2.78	2.23E-01	2.25E-01	2.29E-01
E	0.93	1.04E-01	1.02E-01	1.05E-01
F	0.31	3.98E-02	3.95E-02	4.15E-02
G	0.10	1.39E-02	1.41E-02	1.30E-02
H	0.03		7.00E-04	8.50E-04

Yield (mg/mL): 1.59 Dilution Factor: 100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:36:41.

Thermostability Assay Data

**slope of N421D A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		4.00E-04	4.00E-04
B	48.3	4.00E-04	7.00E-04	1.10E-03
C	45.7	1.50E-03	1.40E-03	1.60E-03
D	42.4	7.10E-02	6.83E-02	6.25E-02
E	37.7	1.47E-01	1.52E-01	1.46E-01
F	33.6	1.57E-01	1.62E-01	1.71E-01
G	31.3	1.73E-01	1.67E-01	1.63E-01
H	30.0	1.57E-01	1.66E-01	1.70E-01

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:16:38.

Reference Data for WT

**slope of N421D A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.0012000	0.000600
B	48.3	0.0011		0.0009000
C	45.7	0.0011	0.001100	0.001200
D	42.4	0.0791	0.09140	0.11770
E	37.7	0.6272	0.5683	0.5244
F	33.6	0.6226	0.6248	0.6226
G	31.3	0.6319	0.6288	0.6255
H	30.0	0.628	0.6750	0.6181

Protein purified on 11/6/2024 and assayed on 11/6/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:53:49.

Design Data

Rosetta Score for N421D: -0.191

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.