Raw Data for BglB Variant S203V

Expression Data

SDS-PAGE
 0.98

Kinetic Assay Data

slope of S203V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3782 0.3927 0.3880
B 25.00 0.3105 0.3181 0.3170
C 8.33 0.2316 0.2410 0.2188
D 2.78 0.2377 0.2078 0.2240
E 0.93 0.07425 0.07465 0.07495
F 0.31 0.02880 0.03025 0.03155
G 0.10 0.010000 0.009750 0.009500
H 0.03 0.004600 0.004000 0.004200
 0.98      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by VarunP and last updated on 2024-11-20 11:53:38.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3217 0.3208 0.3160
B 25.00 0.2632 0.2678 0.2543
C 8.33 0.2580 0.21740 0.1913
D 2.78 0.1417 0.151400 0.1573
E 0.93 0.06825 0.066150 0.06180
F 0.31 0.02710 0.02625 0.02360
G 0.10 0.009200 0.00885 0.007850
H 0.03 0.004150 0.0039 0.003650
 0.82      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:15:24.


Thermostability Assay Data

slope of S203V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00150 0.0012000 0.001600
B 48.3 0.0065000 0.006400 0.0062000
C 45.7 0.109600 0.129000 0.156300
D 42.4 0.37140 0.31000 0.30770
E 37.7 0.3841 0.4101 0.3596
F 33.6 0.3336 0.3337 0.3605
G 31.3 0.3730 0.3750 0.3575
H 30.0 0.4295 0.4175 0.4324

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by VarunP and last updated on 2024-11-20 12:06:57.


Reference Data for WT

slope of S203V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00100 0.000700
B 48.3 0.0006000
C 45.7 0.000900 0.0009
D 42.4 0.04390 0.05580 0.0707
E 37.7 0.2862 0.3131 0.3533
F 33.6 0.2663 0.2985 0.2944
G 31.3 0.2921 0.2913 0.2726
H 30.0 0.3055 0.3273 0.3522

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:22:40.


Design Data

-4.121

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.