Raw Data for BglB Variant C38S

Expression Data

SDS-PAGE
 1.03

Kinetic Assay Data

slope of C38S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3124 0.3203 0.3200
B 25.00 0.2557 0.2611 0.2543
C 8.33 0.1786 0.1741 0.1876
D 2.78
E 0.93 0.06215 0.05735 0.06080
F 0.31 0.02715 0.02615 0.02390
G 0.10 0.008650 0.008850 0.008450
H 0.03 0.004000 0.003650 0.003350
 1.03      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by lucyni and last updated on 2024-11-20 11:45:07.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3217 0.3208 0.3160
B 25.00 0.2632 0.2678 0.2543
C 8.33 0.2580 0.21740 0.1913
D 2.78 0.1417 0.151400 0.1573
E 0.93 0.06825 0.066150 0.06180
F 0.31 0.02710 0.02625 0.02360
G 0.10 0.009200 0.00885 0.007850
H 0.03 0.004150 0.0039 0.003650
 0.82      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:15:24.


Thermostability Assay Data

slope of C38S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00110 0.0011000
B 48.3 0.0008000 0.000900 0.0006000
C 45.7 0.000800 0.001000 0.000900
D 42.4 0.04160 0.04000
E 37.7 0.2492 0.2610 0.2706
F 33.6 0.2352 0.2520
G 31.3 0.2533 0.2739 0.2571
H 30.0 0.2950 0.2875 0.2779

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by lucyni and last updated on 2024-11-20 11:56:02.


Reference Data for WT

slope of C38S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00100 0.000700
B 48.3 0.0006000
C 45.7 0.000900 0.0009
D 42.4 0.04390 0.05580 0.0707
E 37.7 0.2862 0.3131 0.3533
F 33.6 0.2663 0.2985 0.2944
G 31.3 0.2921 0.2913 0.2726
H 30.0 0.3055 0.3273 0.3522

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by ivyliang and last updated on 2024-11-20 12:22:40.


Design Data

1.081

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.