D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V52F

Yield (mg/mL): 0.4571

**slope of V52F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.49E-04	1.02E-03	9.32E-04
B	25.00	7.72E-04	8.66E-04	8.15E-04
C	8.33	5.36E-04	5.25E-04	5.13E-04
D	2.78	3.51E-04	3.86E-04	3.96E-04
E	0.93	1.98E-04	2.12E-04	1.89E-04
F	0.31	1.15E-04	9.51E-05	1.37E-04
G	0.10	5.24E-05	6.97E-05	7.01E-05
H	0.03	5.96E-05	3.23E-05	4.21E-05

Yield (mg/mL): 0.4571 Dilution Factor: 100×

Protein purified on 10/14/24 and assayed on 10/21/24.
Data uploaded by vehrman and last updated on 2024-11-18 12:26:28.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.87E-03	3.86E-03	3.47E-03
B	25.00	2.04E-03	1.97E-03	1.88E-03
C	8.33	1.17E-03	1.18E-03	1.17E-03
D	2.78	7.03E-04	6.74E-04	7.25E-04
E	0.93	3.45E-04	3.09E-04	3.54E-04
F	0.31	1.52E-04	1.37E-04	1.83E-04
G	0.10	8.90E-05	1.16E-04	1.04E-04
H	0.03	2.54E-05	3.53E-05	2.81E-05

Yield (mg/mL): 0.164 Dilution Factor: 100×

Protein purified on 10/14/2024 and assayed on 10/21/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:46:29.

**slope of V52F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		3.09E-05	3.34E-05
B	48.3		2.80E-05	3.10E-05
C	45.7	3.64E-05		3.13E-05
D	42.4	8.74E-05	7.14E-05	8.29E-05
E	37.7	2.82E-04	2.75E-04	3.01E-04
F	33.6	3.79E-04	3.94E-04	3.50E-04
G	31.3	3.85E-04	3.69E-04	3.49E-04
H	30.0	3.82E-04	3.53E-04	2.78E-04

Protein purified on 10/24/24 and assayed on 10/28/24.
Data uploaded by vehrman and last updated on 2024-11-18 12:41:58.

**slope of V52F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	2.01E-05		2.46E-05
B	48.3	3.11E-05	3.01E-05	3.11E-05
C	45.7	3.81E-05	4.19E-05	5.07E-05
D	42.4	3.59E-04	3.22E-04	3.57E-04
E	37.7	6.78E-04	6.82E-04	8.13E-04
F	33.6
G	31.3	8.93E-04		8.75E-04
H	30.0	9.06E-04	9.95E-04	8.85E-04

Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:57:48.

Rosetta Score for V52F: -0.865

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.