D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M149I

Yield (mg/mL): 0.137

**slope of M149I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.89E-03	6.28E-03	5.64E-03
B	25.00	6.01E-03	7.41E-03	6.14E-03
C	8.33	4.05E-03	3.75E-03	4.05E-03
D	2.78	3.57E-03	3.01E-03	3.71E-03
E	0.93	2.84E-03		2.56E-03
F	0.31	2.85E-03	3.99E-03	3.30E-03
G	0.10	2.51E-03	3.21E-03	3.42E-03
H	0.03	2.33E-03	2.55E-03	3.07E-03

Yield (mg/mL): 0.137 Dilution Factor: 100×

Protein purified on 9/25/24 and assayed on 10/21/24.
Data uploaded by isteckler and last updated on 2024-11-18 12:53:12.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.02E-03	3.93E-03	3.66E-03
B	25.00	1.90E-03	1.66E-03	1.93E-03
C	8.33	1.25E-03	1.20E-03	1.21E-03
D	2.78	7.19E-04	7.26E-04	7.14E-04
E	0.93	3.47E-04	4.22E-04	3.45E-04
F	0.31	1.62E-04	1.94E-04	1.81E-04
G	0.10	8.76E-05	1.19E-04	9.07E-05
H	0.03	4.84E-05	5.27E-05	5.71E-05

Yield (mg/mL): 0.164 Dilution Factor: 100×

Protein purified on 10/14/2024 and assayed on 10/21/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:50:28.

**slope of M149I A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	2.18E-03	1.99E-03	2.42E-03
B	48.3	2.13E-03	1.69E-03	1.86E-03
C	45.7	2.71E-03	2.42E-03	2.42E-03
D	42.4	1.23E-03	1.34E-03
E	37.7	2.11E-03	2.27E-03	2.51E-03
F	33.6	2.96E-03	3.58E-03	3.46E-03
G	31.3	2.51E-03		2.67E-03
H	30.0	2.37E-03	2.13E-03

Protein purified on 9/25/24 and assayed on 10/28/24.
Data uploaded by isteckler and last updated on 2024-11-20 11:09:05.

**slope of M149I A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	3.74E-05	3.06E-05	5.33E-05
B	48.3	4.77E-05	3.57E-05	4.54E-05
C	45.7	5.63E-05	6.40E-05	5.84E-05
D	42.4	3.39E-04	3.13E-04	3.34E-04
E	37.7	7.13E-04	7.39E-04	7.44E-04
F	33.6	1.09E-03	8.86E-04	1.04E-03
G	31.3	1.05E-03	9.46E-04	1.11E-03
H	30.0	1.20E-03	1.18E-03	1.09E-03

Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:58:39.

Rosetta Score for M149I: 88.033

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.