Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.164

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.05E-03	2.78E-03	2.77E-03
	B	25.00	1.87E-03	1.64E-03	1.67E-03
	C	8.33	1.14E-03	1.03E-03	1.06E-03
	D	2.78	6.69E-04	6.26E-04	6.06E-04
	E	0.93	3.23E-04	3.20E-04	2.64E-04
	F	0.31	1.27E-04	1.19E-04	1.32E-04
	G	0.10	5.74E-05	6.39E-05	5.94E-05
	H	0.03	5.01E-05	4.44E-05	3.97E-05

Yield (mg/mL): 0.164     Dilution Factor:  100×

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Protein purified on 10/14/2024 and assayed on 10/21/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:41:12.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.57E-05	2.66E-05
	B	48.3		2.46E-05	2.31E-05
	C	45.7	4.04E-05		4.34E-05
	D	42.4	3.14E-04	3.25E-04	3.12E-04
	E	37.7	6.66E-04	5.57E-04	6.69E-04
	F	33.6	7.47E-04	7.14E-04	7.36E-04
	G	31.3	7.41E-04	7.58E-04
	H	30.0	7.98E-04	7.96E-04	8.43E-04

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Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:59:59.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.