Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.164

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.02E-03	3.93E-03	3.66E-03
	B	25.00	1.90E-03	1.66E-03	1.93E-03
	C	8.33	1.25E-03	1.20E-03	1.21E-03
	D	2.78	7.19E-04	7.26E-04	7.14E-04
	E	0.93	3.47E-04	4.22E-04	3.45E-04
	F	0.31	1.62E-04	1.94E-04	1.81E-04
	G	0.10	8.76E-05	1.19E-04	9.07E-05
	H	0.03	4.84E-05	5.27E-05	5.71E-05

Yield (mg/mL): 0.164     Dilution Factor:  100×

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Protein purified on 10/14/2024 and assayed on 10/21/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:50:28.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	3.74E-05	3.06E-05	5.33E-05
	B	48.3	4.77E-05	3.57E-05	4.54E-05
	C	45.7	5.63E-05	6.40E-05	5.84E-05
	D	42.4	3.39E-04	3.13E-04	3.34E-04
	E	37.7	7.13E-04	7.39E-04	7.44E-04
	F	33.6	1.09E-03	8.86E-04	1.04E-03
	G	31.3	1.05E-03	9.46E-04	1.11E-03
	H	30.0	1.20E-03	1.18E-03	1.09E-03

Download these data

Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:58:39.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.