D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L219R

Expression Data

Yield (mg/mL): 3.015

Kinetic Assay Data

**slope of L219R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.66E-01	2.49E-01	2.42E-01
B	25.00	2.09E-01	2.01E-01	2.08E-01
C	8.33	1.90E-01	1.99E-01	1.84E-01
D	2.78	1.19E-01	1.40E-01	1.37E-01
E	0.93	5.35E-02	6.24E-02	5.83E-02
F	0.31	1.86E-02	1.74E-02	1.94E-02
G	0.10	7.30E-03	6.70E-03	7.15E-03
H	0.03	1.50E-04		1.50E-04

Yield (mg/mL): 3.015 Dilution Factor: 100×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:42:07.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.99E-01	5.93E-01	6.85E-01
B	25.00	4.90E-01	5.58E-01	5.92E-01
C	8.33	4.43E-01	4.73E-01	4.36E-01
D	2.78	2.74E-01	3.11E-01	2.38E-01
E	0.93	1.01E-01	1.06E-01	1.13E-01
F	0.31	3.54E-02	4.41E-02	4.00E-02
G	0.10	1.39E-02	1.72E-02	1.47E-02
H	0.03	2.00E-04	2.50E-04

Yield (mg/mL): 6.462 Dilution Factor: 100×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 14:29:47.

Thermostability Assay Data

**slope of L219R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0		0
B	48.3	0		0
C	45.7	0	2.00E-04	4.00E-04
D	42.4	2.10E-03	2.00E-03
E	37.7	4.46E-02	4.33E-02	4.86E-02
F	33.6	9.27E-02	1.04E-01	1.05E-01
G	31.3	9.97E-02	1.13E-01	1.34E-01
H	30.0	1.17E-01	1.13E-01	9.96E-02

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:43:33.

Reference Data for WT

**slope of L219R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	4.50E-04	6.50E-04	8.00E-04
B	48.3	3.00E-04	3.00E-04	3.50E-04
C	45.7	9.50E-04	9.00E-04	9.50E-04
D	42.4	6.20E-02	7.41E-02	7.36E-02
E	37.7	3.77E-01	3.66E-01	3.73E-01
F	33.6	4.14E-01	4.10E-01	4.06E-01
G	31.3	4.03E-01	3.87E-01	4.10E-01
H	30.0	4.03E-01	4.11E-01	3.41E-01

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 14:43:08.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.