Raw Data for BglB Variant M323I

Expression Data

 5.044

Kinetic Assay Data

slope of M323I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.68E-01 4.79E-01 4.72E-01
B 25.00 3.70E-01 3.94E-01 3.72E-01
C 8.33 3.08E-01 3.30E-01 2.98E-01
D 2.78 2.22E-01 2.41E-01 2.19E-01
E 0.93 8.84E-02 1.19E-01 9.90E-02
F 0.31 3.60E-02 4.12E-02 3.86E-02
G 0.10 1.13E-02 1.46E-02 1.30E-02
H 0.03 1.50E-04 3.50E-04 3.00E-04
 5.044      100×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:25:28.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.7025 0.7268 0.7329
B 25.00 0.5354 0.5554 0.5001
C 8.33 0.4335 0.4443 0.4585
D 2.78 0.2829 0.3052 0.3458
E 0.93 0.1691 0.1414 0.1110
F 0.31 0.06150 0.05505 0.04585
G 0.10 0.02380 0.01940 0.01410
H 0.03 0 0 0
 6.462      100×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-13 17:25:05.


Thermostability Assay Data

slope of M323I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0
C 45.7 2.00E-04 1.00E-04 3.00E-04
D 42.4 1.32E-02 1.56E-02 1.84E-02
E 37.7 9.35E-02 6.16E-02
F 33.6 2.83E-01 2.78E-01 2.59E-01
G 31.3 2.73E-01 2.73E-01
H 30.0 2.93E-01 3.14E-01 3.42E-01

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:27:55.


Reference Data for WT

slope of M323I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0003500 0.0003500
B 48.3 0.0003000 0
C 45.7 0.001550 0.001450
D 42.4 0.05926 0.04375 0.07995
E 37.7 0.3619 0.4019
F 33.6 0.4078 0.3644 0.3481
G 31.3 0.4219 0.4234 0.4218
H 30.0 0.4428 0.4017 0.3841

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-13 17:36:02.


Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.