Raw Data for BglB Variant H315C

Expression Data

 0.368

Kinetic Assay Data

slope of H315C A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.50E-04 7.50E-04 8.00E-04
B 25.00 7.00E-04 6.50E-04
C 8.33 4.50E-04 6.00E-04 5.00E-04
D 2.78 3.50E-04 3.00E-04 4.00E-04
E 0.93 2.50E-04 2.00E-04 1.50E-04
F 0.31 1.50E-04 0 2.00E-04
G 0.10 2.00E-04 2.00E-04 1.50E-04
H 0.03 0 0
 0.368      10×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:21:10.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.7025 0.7268 0.7329
B 25.00 0.5354 0.5554 0.5001
C 8.33 0.4335 0.4443 0.4585
D 2.78 0.2829 0.3052 0.3458
E 0.93 0.1691 0.1414 0.1110
F 0.31 0.06150 0.05505 0.04585
G 0.10 0.02380 0.01940 0.01410
H 0.03 0 0 0
 6.462      100×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-13 17:25:05.


Thermostability Assay Data

slope of H315C A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0
C 45.7 2.50E-04 2.00E-04
D 42.4 0 0
E 37.7 3.50E-04 3.00E-04 3.00E-04
F 33.6 3.50E-04 3.00E-04 4.00E-04
G 31.3 4.00E-04 4.00E-04 3.50E-04
H 30.0 5.50E-04 6.00E-04 4.50E-04

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:22:17.


Reference Data for WT

slope of H315C A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0003500 0.0003500
B 48.3 0.0003000 0
C 45.7 0.001550 0.001450
D 42.4 0.05926 0.04375 0.07995
E 37.7 0.3619 0.4019
F 33.6 0.4078 0.3644 0.3481
G 31.3 0.4219 0.4234 0.4218
H 30.0 0.4428 0.4017 0.3841

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-13 17:36:02.


Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.