Raw Data for BglB Variant N220L

Expression Data

SDS-PAGE
 0.874

Kinetic Assay Data

slope of N220L A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.49E-01 1.57E-01 1.59E-01
B 25.00 1.09E-01 1.22E-01 1.34E-01
C 8.33 7.54E-02 9.67E-02 4.94E-02
D 2.78 4.79E-02 4.65E-02 6.00E-02
E 0.93 2.44E-02 2.16E-02 1.95E-02
F 0.31 2.42E-02 2.34E-02 1.88E-02
G 0.10 8.90E-03 9.20E-03 8.50E-03
H 0.03 5.20E-03 4.70E-03 4.40E-03
 0.874      100×

Protein purified on 4/14/2021 and assayed on 4/28/2021.
Data uploaded by czulev01 and last updated on 2023-01-11 13:33:00.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 0.0958 0.0874
C 8.33 0.073 0.0675
D 2.78 0.0535 0.0476
E 0.93 0.0206 0.0189
F 0.31 0.0185 0.0142
G 0.10 0.008 0.0044
H 0.03 0.0041 0.0033
 0.834      100×

Protein purified on 4/20/21 and assayed on 4/20/21.
Data uploaded by aguiri01 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N220L A420 nm (min−1)
T (°C) 1 2
A 30.0 1.07E+00 1.02E+00
B 31.8
C 33.6
D 35.5 0.9945 0.8898
E 37.3 0.8171 0.6758
F 39.1 0.794 0.6608
G 40.9 0.1158 0.116
H 42.7 0.0042 0.0043
I 44.5 0.0003 0.0007
J 46.4 0.0001 0.0002
K 48.1 0.0004 0.0004
L 50.0 0.0002 0.0002

Protein purified on 4/14/2021 and assayed on 4/28/2021.
Data uploaded by czulev01 and last updated on 2022-07-28 15:06:13.


Reference Data for WT

slope of N220L A420 nm (min−1)
T (°C) 1 2
A 30.0 0.6649
B 31.8 0.7928
C 33.6 0.7785
D 35.5 0.6394
E 37.3 0.7241
F 39.1 0.6772
G 40.9 0.2588
H 42.7 0.0309
I 44.5 0.0012
J 46.4 0.0004
K 48.1 0.0003
L 50.0 0.0002

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-07-28 16:00:19.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.