D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L434S

Expression Data

Yield (mg/mL): 0.967

Kinetic Assay Data

**slope of L434S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0623	0.0548	0.0552
B	25.00	0.056	0.0492	0.0495
C	8.33	0.047	0.0433	0.0424
D	2.78	0.0308	0.0281	0.0293
E	0.93	0.01535	0.0137	0.01415
F	0.31	0.006	0.00565	0.00555
G	0.10	0.0023	0.00225	0.00235
H	0.03	0.00035	0.0003	0.00025

Yield (mg/mL): 0.967 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by jmpender and last updated on 2024-10-22 21:15:24.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1193	0.1123	0.1084
B	25.00	0.1097	0.0945	0.0935
C	8.33	0.0857	0.0748	0.0739
D	2.78	0.0490	0.0457	0.0472
E	0.93	0.02215	0.02030	0.02075
F	0.31	0.00840	0.00820	0.00815
G	0.10	0.003250	0.003200	0.003150
H	0.03	0.000150	0.000300	0.000200

Yield (mg/mL): 1.517 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by jmpender and last updated on 2024-10-22 20:00:54.

Design Data

Rosetta Score for L434S: 1.195

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.