Raw Data for BglB Variant H315D

Expression Data

SDS-PAGE
 0.316

Kinetic Assay Data

slope of H315D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.16E-02 9.09E-02 9.59E-02
B 25.00 7.39E-02 8.16E-02 8.24E-02
C 8.33 7.64E-02 7.03E-02 7.32E-02
D 2.78 6.54E-02 5.62E-02
E 0.93 3.95E-02 4.73E-02
F 0.31 3.45E-02 3.66E-02 3.27E-02
G 0.10
H 0.03 3.07E-02 2.99E-02 2.28E-02
 0.316      100×

Protein purified on 4/21/21 and assayed on 4/28/21.
Data uploaded by csoderman and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.03E-01 1.02E-01
B 25.00 9.10E-02 9.64E-02
C 8.33 8.28E-02 8.23E-02
D 2.78 6.68E-02 5.44E-02
E 0.93 6.22E-02 4.12E-02
F 0.31 5.06E-02 4.04E-02
G 0.10
H 0.03 3.66E-02 2.16E-02
 0.834      100×

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by csoderman and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of H315D A420 nm (min−1)
T (°C) 1 2
A 30.0 6.03E-01 6.73E-01
B 31.8 6.69E-01 6.61E-01
C 33.6 6.61E-01 6.69E-01
D 35.5 0.6569 0.6414
E 37.3 0.6431 0.6365
F 39.1 0.5541 0.5367
G 40.9 0.1515 0.1721
H 42.7 0.0048 0.0061
I 44.5 0.0006 0.0008
J 46.4 0.0002 0.0008
K 48.1
L 50.0 0.0005

Protein purified on 4/21/21 and assayed on 4/28/21.
Data uploaded by csoderman and last updated on 2022-07-28 15:06:03.


Reference Data for WT

slope of H315D A420 nm (min−1)
T (°C) 1 2
A 30.0 4.648E-01 4.283E-01
B 31.8 4.733E-01 4.543E-01
C 33.6 4.575E-01 4.692E-01
D 35.5 4.611E-01 4.592E-01
E 37.3 4.559E-01 4.536E-01
F 39.1 3.877E-01 4.000E-01
G 40.9 1.069E-01 1.150E-01
H 42.7 4.025E-03 3.058E-03
I 44.5 1.635E-04 4.815E-04
J 46.6 0 2.675E-04
K 48.1 2.794E-04 6.750E-05
L 50.0 3.738E-04 1.060E-04

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-07-28 13:43:07.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.