D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A408K

Expression Data

Yield (mg/mL): 2.88

Kinetic Assay Data

**slope of A408K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.73E-01	2.68E-01	2.70E-01
B	25.00	2.12E-01	2.16E-01	2.05E-01
C	8.33	1.78E-01	1.73E-01	1.73E-01
D	2.78	1.07E-01	1.12E-01	1.12E-01
E	0.93	6.22E-02	6.28E-02	5.89E-02
F	0.31	2.55E-02	2.69E-02	2.35E-02
G	0.10	9.10E-03	9.95E-03	8.85E-03
H	0.03		2.50E-04	2.50E-04

Yield (mg/mL): 2.88 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/8/2024.
Data uploaded by Selena and last updated on 2024-10-16 16:56:51.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.92E-01	1.49E-01	1.98E-01
B	25.00	1.58E-01	1.59E-01	1.59E-01
C	8.33	1.08E-01	1.21E-01	1.25E-01
D	2.78	7.51E-02	7.38E-02	7.73E-02
E	0.93	3.71E-02	3.65E-02	3.96E-02
F	0.31	1.47E-02	1.38E-02	1.56E-02
G	0.10	5.35E-03	4.90E-03	6.10E-03
H	0.03	0	0	0

Yield (mg/mL): 2.092 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/8/2024.
Data uploaded by Selena and last updated on 2024-10-16 17:00:54.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.