Raw Data for BglB Variant P165A

Expression Data

SDS-PAGE
 0.2

Kinetic Assay Data

slope of P165A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0301 0.0298 0.0294
B 25.00 0.0323 0.03 0.0305
C 8.33 0.0204 0.022 0.0238
D 2.78 0.0124 0.0157 0.0146
E 0.93 0.0053 0.0062 0.0064
F 0.31 0.0025 0.0025 0.0027
G 0.10 0.0008 0.0007 0.0009
H 0.03 0.0001 0.0001
 0.2      100×

Protein purified on 4/21/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.132 0.1296
B 25.00 0.1202 0.1221
C 8.33 0.0805 0.0851
D 2.78 0.0499 0.0523
E 0.93 0.0228 0.0224
F 0.31 0.0085 0.0084
G 0.10 0.0037 0.0034
H 0.03 0.0014 0.0014
 0.834      100×

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of P165A A420 nm (min−1)
T (°C) 1 2
A 30.0 0.2094 0.2
B 31.8
C 33.6 0.2125 0.2217
D 35.5 0.2431 0.239
E 37.3 0.2104 0.1377
F 39.1 0.1526 0.1189
G 40.9 0.0517 0.0553
H 42.7 0.0028 0.0027
I 44.5 0.0006 0.0003
J 46.4 0.0006 0.0003
K 48.1 0.0002 0.0003
L 50.0 0.0001 0.0001

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-07-28 15:46:26.


Reference Data for WT

slope of P165A A420 nm (min−1)
T (°C) 1 2
A 30.0 0.6649
B 31.8 0.7928
C 33.6 0.7785
D 35.5 0.6394
E 37.3 0.7241
F 39.1 0.6772
G 40.9 0.2588
H 42.7 0.0309
I 44.5 0.0012
J 46.4 0.0004
K 48.1 0.0003
L 50.0 0.0002

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-07-28 16:00:19.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.