D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H223S

Expression Data

Yield (mg/mL): 4.6

Kinetic Assay Data

**slope of H223S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3381	0.3191	0.3374
B	25.00	0.2481	0.2755	0.2276
C	8.33	0.1592	0.1593	0.1764
D	2.78	0.1319	0.1285	0.1214
E	0.93	0.07155	0.05695	0.05470
F	0.31	0.02120	0.02090	0.02125
G	0.10	0.008900	0.006850	0.007500
H	0.03	0	0.0003500	0.0002500

Yield (mg/mL): 4.6 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by ttylam and last updated on 2024-10-07 14:38:00.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3829	0.4375	0.4025
B	25.00	0.3373	0.3516	0.3016
C	8.33	0.2148	0.2543	0.2200
D	2.78	0.1821	0.1617	0.1675
E	0.93	0.06415	0.08460	0.08690
F	0.31	0.02565	0.03030	0.03640
G	0.10	0.009350	0.01060	0.01040
H	0.03		0.0003000	0.0003000

Yield (mg/mL): 4.3 Dilution Factor: 100×

Protein purified on 9/10/2024 and assayed on 9/10/2024.
Data uploaded by ttylam and last updated on 2024-10-07 13:56:17.

Design Data

Rosetta Score for H223S: 1.173

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.