Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.723163529	2.869
	B	25.00	1.896734118	1.882
	C	8.33	0.730718824	2.008
	D	2.78	1.120376471	1.173
	E	0.93	0.976065882	0.791
	F	0.31	0.338332941	0.334
	G	0.10	0.123663529	0.087
	H	0.03	0.109577647	0.078

Yield (mg/mL): 0.819     Dilution Factor:  10×

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Protein purified on and assayed on .
Data uploaded by juliaclevinger and last updated on 2024-06-07 13:35:37.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	15.95725714
	B	31.8	19.02648
	C	33.6	18.68496
	D	35.5	15.34662857
	E	37.3	17.3784
	F	39.1	16.25304
	G	40.9	6.210704176
	H	42.7	0.742570588	0.346750588
	I	44.5	0.028729412	0.016831765
	J	46.4	0.010468235	0.008643529
	K	48.1	0.006194118	0.006158824
	L	50.0	0.005431765	0.006162353

Download these data

Protein purified on and assayed on .
Data uploaded by juliaclevinger and last updated on 2022-07-28 14:05:21.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.