D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177T

Expression Data

Yield (mg/mL): 2.102

Kinetic Assay Data

**slope of E177T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.61E-01	1.68E-01	1.60E-01
B	25.00	1.14E-01	1.19E-01	1.03E-01
C	8.33	8.28E-02	7.84E-02	8.31E-02
D	2.78	6.55E-02	5.85E-02	6.27E-02
E	0.93	3.35E-02	2.62E-02	2.67E-02
F	0.31	1.39E-02	1.03E-02	1.08E-02
G	0.10	3.90E-03	3.65E-03	3.75E-03
H	0.03	2.00E-04	2.00E-04

Yield (mg/mL): 2.102 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/4/2024.
Data uploaded by Selena and last updated on 2024-09-18 14:10:15.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.36E-01	1.44E-01	2.79E-01
B	25.00	2.27E-01	2.04E-01	1.50E-01
C	8.33	1.49E-01	1.46E-01	1.66E-01
D	2.78	9.26E-02	9.48E-02	1.20E-01
E	0.93	3.10E-02	4.24E-02	5.35E-02
F	0.31	1.34E-02	1.67E-02
G	0.10	5.15E-03	5.50E-03
H	0.03	0	0	0

Yield (mg/mL): 2.102 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/4/2024.
Data uploaded by Selena and last updated on 2024-09-18 14:29:18.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.