D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A236C

Expression Data

Yield (mg/mL): 1.45

Kinetic Assay Data

**slope of A236C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.31E-02	3.28E-02	3.27E-02
B	25.00	3.06E-02	2.58E-02	2.29E-02
C	8.33	1.48E-02	1.50E-02	1.59E-02
D	2.78	1.06E-02	1.01E-02	9.75E-03
E	0.93	6.85E-03	5.35E-03	4.70E-03
F	0.31	1.85E-03	2.15E-03	2.00E-03
G	0.10	9.50E-04	1.00E-03	9.50E-04
H	0.03	1.50E-04

Yield (mg/mL): 1.45 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/5/2024.
Data uploaded by jishy7 and last updated on 2024-09-18 13:53:46.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.21E-01	1.06E-01
B	25.00	9.95E-02	8.89E-02	9.32E-02
C	8.33	7.30E-02	6.83E-02	7.14E-02
D	2.78	4.39E-02	4.79E-02	4.88E-02
E	0.93	1.93E-02	2.16E-02	2.27E-02
F	0.31	7.10E-03	7.20E-03	7.90E-03
G	0.10	2.60E-03	2.95E-03	3.15E-03
H	0.03		3.00E-04

Yield (mg/mL): 2.3 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/5/2024.
Data uploaded by jishy7 and last updated on 2024-09-18 13:42:55.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.