Raw Data for BglB Variant F253S

Expression Data

 0.819

Kinetic Assay Data

slope of F253S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.647 2.503 3.165
B 25.00 2.660 2.773 2.678
C 8.33 2.457 2.058 2.543
D 2.78 1.761 1.677 1.342
E 0.93 1.224 1.214
F 0.31 0.598 0.520 0.327
G 0.10 0.267 0.233 0.177
H 0.03 0.120 0.101 0.065
 0.819      100×

Protein purified on and assayed on .
Data uploaded by juliaclevinger and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.723163529 2.869
B 25.00 1.896734118 1.882
C 8.33 0.730718824 2.008
D 2.78 1.120376471 1.173
E 0.93 0.976065882 0.791
F 0.31 0.338332941 0.334
G 0.10 0.123663529 0.087
H 0.03 0.109577647 0.078
 0.819      10×

Protein purified on and assayed on .
Data uploaded by juliaclevinger and last updated on 2024-06-07 13:35:37.


Thermostability Assay Data

slope of F253S A420 nm (min−1)
T (°C) 1 2
A 30.0 12.86365714 13.30551429
B 31.8 14.47872 13.34528571
C 33.6 18.27024 16.79664
D 35.5 19.95648 18.26232
E 37.3 14.80649143 14.13085714
F 39.1 14.36386286 14.75650286
G 40.9 2.936915294 2.888654118
H 42.7 0.088715294 0.071385882
I 44.5 0.004535294 0.004249412
J 46.4 0.006222353 0.003049412
K 48.1 0.000172941 0.003490588
L 50.0 0.002354118 0.002198824

Protein purified on and assayed on .
Data uploaded by juliaclevinger and last updated on 2022-07-28 14:05:14.


Reference Data for WT

slope of F253S A420 nm (min−1)
T (°C) 1 2
A 30.0 15.95725714
B 31.8 19.02648
C 33.6 18.68496
D 35.5 15.34662857
E 37.3 17.3784
F 39.1 16.25304
G 40.9 6.210704176
H 42.7 0.742570588 0.346750588
I 44.5 0.028729412 0.016831765
J 46.4 0.010468235 0.008643529
K 48.1 0.006194118 0.006158824
L 50.0 0.005431765 0.006162353

Protein purified on and assayed on .
Data uploaded by juliaclevinger and last updated on 2022-07-28 14:05:21.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.