Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.12E-01	1.19E-01
	B	25.00	9.11E-02	9.53E-02
	C	8.33	8.12E-02	8.28E-02
	D	2.78	5.24E-02	3.59E-02
	E	0.93	2.76E-02	2.27E-02
	F	0.31	1.25E-02	9.84E-03
	G	0.10	3.03E-03	3.03E-03
	H	0.03	5.47E-04	1.28E-03

Yield (mg/mL): 0.834     Dilution Factor:  100×

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Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	4.648E-01	4.283E-01
	B	31.8	4.733E-01	4.543E-01
	C	33.6	4.575E-01	4.692E-01
	D	35.5	4.611E-01	4.592E-01
	E	37.3	4.559E-01	4.536E-01
	F	39.1	3.877E-01	4.000E-01
	G	40.9	1.069E-01	1.150E-01
	H	42.7	4.025E-03	3.058E-03
	I	44.5	1.635E-04	4.815E-04
	J	46.6	0	2.675E-04
	K	48.1	2.794E-04	6.750E-05
	L	50.0	3.738E-04	1.060E-04

Download these data

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-07-28 13:43:07.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.