D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K413A

Expression Data

Yield (mg/mL): 3.15

Kinetic Assay Data

**slope of K413A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.21E-01	3.38E-01	3.10E-01
B	25.00	2.79E-01	2.91E-01	2.84E-01
C	8.33	1.76E-01	1.73E-01	1.86E-01
D	2.78	8.98E-02	9.32E-02	9.47E-02
E	0.93	5.98E-02	6.08E-02	6.56E-02
F	0.31	2.56E-02	2.36E-02	2.78E-02
G	0.10	7.35E-03	7.15E-03	9.35E-03
H	0.03	2.50E-04	2.50E-04	2.50E-04

Yield (mg/mL): 3.15 Dilution Factor: 100×

Protein purified on 7/29/24 and assayed on 8/1/24.
Data uploaded by Sophie and last updated on 2024-09-03 17:09:07.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.22E-01	3.64E-01	3.49E-01
B	25.00	2.46E-01	3.03E-01	2.86E-01
C	8.33	1.72E-01	1.85E-01	1.88E-01
D	2.78	1.14E-01	9.07E-02	1.04E-01
E	0.93	6.08E-02	6.57E-02	8.43E-02
F	0.31	2.31E-02	2.83E-02	2.77E-02
G	0.10	7.45E-03	9.30E-03	1.03E-02
H	0.03	2.50E-04	3.00E-04	2.00E-04

Yield (mg/mL): 2 Dilution Factor: 100×

Protein purified on 7/29/24 and assayed on 8/1/24.
Data uploaded by Sophie and last updated on 2024-08-21 11:02:46.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.