D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61S

Expression Data

Yield (mg/mL): 3.066

Kinetic Assay Data

**slope of K61S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.79E-01	2.98E-01	2.80E-01
B	25.00	2.06E-01	2.17E-01	2.08E-01
C	8.33	1.94E-01	2.03E-01	1.83E-01
D	2.78	1.17E-01	1.28E-01	1.10E-01
E	0.93	5.15E-02	5.05E-02	4.55E-02
F	0.31	1.80E-02	1.95E-02	1.80E-02
G	0.10	7.00E-03	7.00E-03	7.00E-03
H	0.03	1.00E-03	1.00E-03	1.00E-03

Yield (mg/mL): 3.066 Dilution Factor: 100×

Protein purified on 4/11/2023 and assayed on 4/13/2023.
Data uploaded by mspiridonova and last updated on 2024-09-29 18:35:20.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.00E-01	7.42E-01	8.28E-01
B	25.00	6.01E-01	6.25E-01	6.55E-01
C	8.33	5.00E-01	4.69E-01	5.05E-01
D	2.78	3.21E-01	3.51E-01	3.74E-01
E	0.93	1.48E-01	1.40E-01	1.62E-01
F	0.31	5.70E-02	5.80E-02	6.75E-02
G	0.10	2.10E-02	2.05E-02	2.20E-02
H	0.03	1.00E-03		1.00E-03

Yield (mg/mL): 7.569 Dilution Factor: 100×

Protein purified on 4/11/2023 and assayed on 4/13/2023.
Data uploaded by mspiridonova and last updated on 2024-09-29 19:16:59.

Design Data

Rosetta Score for K61S: 2.067

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.