Raw Data for BglB Variant C49S

Expression Data

SDS-PAGE
 0.851

Kinetic Assay Data

slope of C49S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.17E-01 1.58E-01 5.38E-02
B 25.00 1.19E-01 1.06E-01 8.26E-02
C 8.33 6.17E-02 6.95E-02
D 2.78 4.62E-02 5.31E-02 5.27E-02
E 0.93 3.10E-02 3.45E-02 2.71E-02
F 0.31 1.27E-02 1.54E-02 1.23E-02
G 0.10 4.12E-03 3.52E-03 2.90E-03
H 0.03 1.35E-03 1.38E-03
 0.851      100×

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.12E-01 1.19E-01
B 25.00 9.11E-02 9.53E-02
C 8.33 8.12E-02 8.28E-02
D 2.78 5.24E-02 3.59E-02
E 0.93 2.76E-02 2.27E-02
F 0.31 1.25E-02 9.84E-03
G 0.10 3.03E-03 3.03E-03
H 0.03 5.47E-04 1.28E-03
 0.834      100×

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of C49S A420 nm (min−1)
T (°C) 1 2
A 30.0 1.13604E+00 1.07778E+00
B 31.8 1.10687E+00 1.09814E+00
C 33.6 1.10606E+00 1.10742E+00
D 35.5 1.12120E+00 1.12645E+00
E 37.3 1.08590E+00 1.07920E+00
F 39.1 8.96850E-01 9.20720E-01
G 40.9 2.38056E-01 2.14745E-01
H 42.7 7.51382E-03 5.84309E-03
I 44.5 5.62206E-04 6.57353E-04
J 46.6 7.13529E-04 4.17353E-04
K 48.1 2.87647E-04 2.69265E-04
L 50.0 2.27206E-04 4.00735E-04

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-07-28 13:43:02.


Reference Data for WT

slope of C49S A420 nm (min−1)
T (°C) 1 2
A 30.0 4.648E-01 4.283E-01
B 31.8 4.733E-01 4.543E-01
C 33.6 4.575E-01 4.692E-01
D 35.5 4.611E-01 4.592E-01
E 37.3 4.559E-01 4.536E-01
F 39.1 3.877E-01 4.000E-01
G 40.9 1.069E-01 1.150E-01
H 42.7 4.025E-03 3.058E-03
I 44.5 1.635E-04 4.815E-04
J 46.6 0 2.675E-04
K 48.1 2.794E-04 6.750E-05
L 50.0 3.738E-04 1.060E-04

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-07-28 13:43:07.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.