D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302A

Expression Data

Yield (mg/mL): 2.163

Kinetic Assay Data

**slope of S302A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.14E-01	2.21E-01	2.15E-01
B	25.00	1.91E-01	1.85E-01	1.80E-01
C	8.33	1.46E-01	1.38E-01	1.35E-01
D	2.78	9.12E-02	8.34E-02	8.18E-02
E	0.93	4.00E-02	3.79E-02	3.70E-02
F	0.31	1.58E-02	1.49E-02	1.46E-02
G	0.10	5.70E-03	5.25E-03	5.25E-03
H	0.03	2.10E-03	1.95E-03	2.00E-03

Yield (mg/mL): 2.163 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 17:00:41.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.73E-01	1.65E-01	1.61E-01
B	25.00	1.44E-01	1.37E-01	1.34E-01
C	8.33	1.08E-01	1.02E-01	1.01E-01
D	2.78	7.04E-02	6.09E-02	6.13E-02
E	0.93	3.23E-02	2.91E-02	2.80E-02
F	0.31	1.26E-02	1.14E-02	1.07E-02
G	0.10	4.20E-03	3.90E-03	3.85E-03
H	0.03	1.65E-03	1.45E-03	1.40E-03

Yield (mg/mL): 1.727 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:58:44.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.