D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y173L

Expression Data

Yield (mg/mL): 0.547

Kinetic Assay Data

**slope of Y173L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	1.35E-02	1.44E-02	1.42E-02
C	8.33	1.16E-02	1.19E-02	1.20E-02
D	2.78	7.85E-03	7.55E-03	7.70E-03
E	0.93	3.80E-03	3.50E-03	3.70E-03
F	0.31	1.60E-03	1.55E-03	1.60E-03
G	0.10	7.00E-04	7.00E-04	7.50E-04
H	0.03		4.00E-04	4.50E-04

Yield (mg/mL): 0.547 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:55:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.27E-01	1.29E-01
B	25.00		1.07E-01	1.06E-01
C	8.33	8.57E-02	7.72E-02	8.17E-02
D	2.78	5.20E-02	4.64E-02	4.79E-02
E	0.93	2.04E-02	2.07E-02	2.18E-02
F	0.31	8.15E-03	8.10E-03	8.60E-03
G	0.10	3.30E-03	2.95E-03	3.10E-03
H	0.03	1.20E-03	1.15E-03	1.25E-03

Yield (mg/mL): 1.575 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:46:19.

Design Data

Rosetta Score for Y173L: 10.067

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.