D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L171I

Expression Data

Yield (mg/mL): 0.655

Kinetic Assay Data

**slope of L171I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.79E-02	3.73E-02
B	25.00	3.54E-02	3.24E-02	3.36E-02
C	8.33	2.54E-02	2.45E-02	2.30E-02
D	2.78	1.52E-02	1.42E-02	1.37E-02
E	0.93	6.55E-03	6.55E-03	6.40E-03
F	0.31	2.50E-03	2.50E-03	2.40E-03
G	0.10	1.00E-03	1.05E-03
H	0.03	5.00E-04	5.50E-04	5.00E-04

Yield (mg/mL): 0.655 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:04:27.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.39E-01	1.32E-01
B	25.00	1.24E-01	1.16E-01	1.11E-01
C	8.33	9.65E-02	8.68E-02	8.47E-02
D	2.78	5.46E-02	5.19E-02	5.04E-02
E	0.93	2.35E-02	2.30E-02	2.32E-02
F	0.31	8.60E-03	8.85E-03	8.80E-03
G	0.10	3.05E-03	3.05E-03	3.10E-03
H	0.03	1.25E-03	1.15E-03	1.20E-03

Yield (mg/mL): 1.719 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 15:46:25.

Design Data

Rosetta Score for L171I: 39.302

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.