Raw Data for BglB Variant W409F

Expression Data

SDS-PAGE
 0.783

Kinetic Assay Data

slope of W409F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1408 0.1370 0.1471
B 25.00 0.1157 0.1193 0.1149
C 8.33 0.1018 0.1000 0.1038
D 2.78 0.0666 0.0656 0.0524
E 0.93 0.0226 0.0373 0.0276
F 0.31 0.0132 0.0134 0.0125
G 0.10 0.0035 0.0042 0.0033
H 0.03 0.0011 0.0012
 0.783      100×

Protein purified on 4/21/2021 and assayed on 4/28/2021.
Data uploaded by herrka01 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.12E-01 1.19E-01
B 25.00 9.11E-02 9.53E-02
C 8.33 8.12E-02 8.28E-02
D 2.78 5.24E-02 3.59E-02
E 0.93 2.76E-02 2.27E-02
F 0.31 1.25E-02 9.84E-03
G 0.10 3.03E-03 3.03E-03
H 0.03 5.47E-04 1.28E-03
 0.834      100×

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of W409F A420 nm (min−1)
T (°C) 1 2
A 30.0 0.9858 0.9632
B 31.8 1.0157 0.9504
C 33.6 1.0004 0.9847
D 35.5 0.9761 0.8888
E 37.3 0.9216 0.9221
F 39.1 0.8135 0.8149
G 40.9 0.1687 0.1160
H 42.7 0.0052 0.0055
I 44.5 0.0005 0.0003
J 46.4 0.0005 0.0002
K 48.1 0.0004 0.0002
L 50.0 0.0001 0.0001

Protein purified on 4/21/2021 and assayed on 4/28/2021.
Data uploaded by herrka01 and last updated on 2022-07-28 15:05:59.


Reference Data for WT

slope of W409F A420 nm (min−1)
T (°C) 1 2
A 30.0 4.648E-01 4.283E-01
B 31.8 4.733E-01 4.543E-01
C 33.6 4.575E-01 4.692E-01
D 35.5 4.611E-01 4.592E-01
E 37.3 4.559E-01 4.536E-01
F 39.1 3.877E-01 4.000E-01
G 40.9 1.069E-01 1.150E-01
H 42.7 4.025E-03 3.058E-03
I 44.5 1.635E-04 4.815E-04
J 46.6 0 2.675E-04
K 48.1 2.794E-04 6.750E-05
L 50.0 3.738E-04 1.060E-04

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-07-28 13:43:07.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.