D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315C

Expression Data

Yield (mg/mL): 5.858

Kinetic Assay Data

**slope of H315C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5559	0.5798	0.5815
B	25.00	0.4609	0.4969	0.4845
C	8.33	0.3543	0.3944	0.3933
D	2.78	0.2681	0.2814	0.2950
E	0.93	0.1203	0.1403	0.1342
F	0.31	0.04980	0.05740	0.05740
G	0.10	0.01625	0.01770	0.01735
H	0.03		0.0002000	0.0001500

Yield (mg/mL): 5.858 Dilution Factor: 100×

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by srumjahn and last updated on 2024-08-06 11:50:10.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5291	0.5354	0.5045
B	25.00	0.4351	0.4413	0.4398
C	8.33	0.3177	0.3323	0.3207
D	2.78	0.2377	0.2274	0.2310
E	0.93	0.08635	0.09780	0.09045
F	0.31	0.04265	0.03885	0.04005
G	0.10	0.01505	0.01610	0.01475
H	0.03	0.0002500		0.0003000

Yield (mg/mL): 5.058 Dilution Factor: 100×

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by srumjahn and last updated on 2024-08-06 11:47:09.

Design Data

Rosetta Score for H315C: 2.611

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.