D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y118A

Expression Data

Yield (mg/mL): 0.31

Kinetic Assay Data

**slope of Y118A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.45E-03	8.95E-03	9.00E-03
B	25.00	6.30E-03	7.10E-03	6.30E-03
C	8.33	4.05E-03	4.15E-03	4.15E-03
D	2.78	2.40E-03	2.30E-03	2.20E-03
E	0.93	1.05E-03	1.45E-03	9.00E-04
F	0.31	4.50E-04		5.00E-04
G	0.10	4.50E-04	5.00E-04	3.50E-04
H	0.03	2.00E-04	0	3.00E-04

Yield (mg/mL): 0.31 Dilution Factor: 10×

Protein purified on 7/17/24 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-01 13:07:06.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.66E-01	1.65E-01	1.67E-01
B	25.00	1.25E-01	1.27E-01	1.21E-01
C	8.33	9.05E-02	9.22E-02	9.42E-02
D	2.78	5.56E-02	5.55E-02	5.69E-02
E	0.93	2.58E-02	2.57E-02	2.57E-02
F	0.31	1.00E-02	9.75E-03	9.70E-03
G	0.10	3.40E-03	3.35E-03	3.60E-03
H	0.03		1.40E-03	1.30E-03

Yield (mg/mL): 1.556 Dilution Factor: 100×

Protein purified on 7/17/24 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-01 12:57:30.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.